SUGAR-LUFF-SCHOORL METHOD 1: Scope and Field of Application This method is for the determination of glucose, reducing sugars expressed as glucose. This adaptation of the Schoorl method was designed for the determination of total The method is based on the Czech Standard ČSN 56 , Český. International Starch Institute Science Park Aarhus, Denmark ISI e Determination of Reducing Sugar, DE by Luff-Schoorl’s method.
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Make up to ml with water and homogenise. The table below is given as a guide for a sample of about 10 g. Purpose and scope This method makes it possible to determine the amount of mustard oil contained in cakes made from the Brassica kuff Sinapis species, and in compound feeding-stuffs which contain cakes made from those species, and, expressed as allyl isothiocyanate, which can be steam entrained.
Cancel Add to project. Use the same volume of water for metohd control test. Procedure Weigh out to the nearest mg approximately 5 g of the sample 2 75 in the case of products which have a tendency to swell and place in a crucible for ashing which has first been calcined and tared. If you want to include your highlights and annotations you must: If the product contains organic matter it is clarified.
The accuracy of the determination of small amounts of reducing sugars, as in refined sugars, has been shown to be very high and comparable with those obtained with the Ofner method. Distil to ml of liquid by passing a powerful current of steam through the flask and gently heating the oil-bath. It is not considered necessary to mention all the apparatus or instruments which form part of the everyday equipment of test laboratories.
Ash the filter and its contents in the original crucible. Chapter 3 Volume 3 P. Please use quotation marks for searching phrases e.
The Luff-Schoorl method for determination of reducing sugar in juices, molasses and sugar
Carry out a blank test in the same way with 5 ml of yeast suspension 3. Chemicals and Chemical Groups see more details. Measure by flame photometry at a wavelength of nm. After cooling transfer the ash quantitatively mefhod a ml graduated flask, using to ml of water and then 50 ml of hydrochloric acid 3. Cool the tube immediately in ice-water and leave there for thirty minutes. Use the Advanced search.
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Cool immediately in cold water and luff approximately five minutes titrate as follows:. Turn the cock 5 in order to connect up tubes 1 and 3 and check that the level is at zero. Decant if necessary centrifugeremove ml of the supernatant liquid and transfer to a ml measuring flask. Make up to volume with water, shake and filter. Purpose and Scope The method makes it possible to determine the total calcium content of feeding-stuffs. Glass filter crucibles of G4 porosity.
Dry and ash as described under 5.
Calibration curve Remove volumes of 1, 2, 4, 5 and 10 ml of the urea solution 3. Techniques, Achoorl and Equipment see more detailsidentification identification Subject Category: Continue calcining luff drying in the oven. If necessary, make up to 25 ml with distilled water and determine the content of reducing sugars by the Luff-Schoorl method. Add 20 ml of silver nitrate solution 3. Zucker 29 6 Whereas the measures provided for in this Directive are in accordance with the Opinion of the Lucf Committee meyhod Feeding-stuffs.
Add to the ammoniacal solution 50 ml of silver nitrate solution 0 71 N 3. Check that there is no hydrocyanic acid in 10 ml of the suspension using sodium picrote paper or by carrying out a blank test as described in the last paragraph of 5.
In the case of higher levels of potassium dilute the solution to be analysed in suitable proportions before adding the loading agent. After cooling to room temperature, make up to the mark with water, shake and filter. Using a burette, transfer the silver nitrate solution 3. Chemicals and Chemical Groups see more detailssucrose sucrose Subject Category: Author Author Serial Subject.
In order to determine the amount of total sugars, continue as described under 5. Don’t have an account?
This method makes it possible to determine the level in feeding-stuffs of mineral substances which are insoluble in hydrochloric acid. Continuing to use www. In the case of feeding-stuffs which are rich in molasses and lufg feeding-stuffs which are not particularly homogeneous, weigh out 20 g and place with ml of water in a 1 litre volumetric flask. Zucker 25 2 In the case of oils and fats, weigh accurately a sample of approximately 25 g in a suitably sized crucible.
Using a pipette, transfer to lyff Erlenmayer flask from 25 ml to ml of the filtrate according to the assumed chlorine content obtained as described under 5. Procedure Crush about 10 g of the sample for example in a coffee mill so that it passes through a sieve with a mesh of 0 72 mm.
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The Luff-Schoorl method of determination of reducing sugars has been universally adapted for the analysis of all boiling house products. The flame shall be regulated in such a way that only the base of the Erlenmeyer is heated. The accuracy of sxhoorl determination of small amounts of reducing sugars, as in refined sugars, has been shown to be very high and comparable with those obtained Purpose and Scope This method makes it possible to determine the crude ash content of feeding-stuffs. Neutralise very slowly with ammonia 3.
schoorl The urea content of the filtrate is determined after the addition of 4-dimethylaminobenzaldehyde 4-DMAB by measuring the optical density at a wavelength of nm, 3. Make up to ml with water.